Fig. 3

CDR1as directly binds with the DBD region of p53 and disrupts the p53/MDM2 complex. a A schema showing four constructs containing full-length or different domains of p53. b RIP-qPCR analysis of CDR1as binding with the indicated p53 constructs. c IP analysis of interaction between MDM2 and p53 in U87MG cells transfected with increasing concentrations of plasmid encoding CDR1as. d IP analysis of interaction between MDM2 and p53 in U87MG cells co-transfected with plasmids encoding CDR1as, Myc-p53 or Myc-MDM2. e-h IP analysis of interaction between MDM2 and the indicated p53 constructs in MEF DKO (p53^−/−; MDM2^−/−) cells co-transfected with plasmids encoding CDR1as, HA-MDM2, and the indicated p53 constructs. *p < 0.05; **p < 0.01