Fig. 2
From: Circular HER2 RNA positive triple negative breast cancer is sensitive to Pertuzumab
Circ-HER2 is enriched in part of TNBC. a The relative expression levels of circ-HER2 determined by q-PCR using junction specific primers in mammary normal cell lines (MCF10A) and different types of breast cancer cell lines as indicated. b The relative expression levels of circ-HER2 in 23 paired HER2 positive breast cancer specimens and the corresponding paired normal adjacent tissues was determined by using junction specific primers. c Left, the relative expression levels of circ-HER2 in 59 paired TNBC specimens and the corresponding paired normal adjacent tissues was determined by using junction specific primers; right, the ratio of circ-HER2 enrichment in 59 TNBC tissues. d Left, the relative expression levels of linear HER2 in 59 paired TNBC specimens and the corresponding paired normal adjacent tissues was determined by using linear specific primers. Right, the relative expression levels of linear HER2 in TNBC tissues from TCGA database. e Kaplan-Meier analysis of the correlation between circ-HER2 RNA expression and overall survival in the 59 TNBC patient’s cohort. Lines show the mean ± SD, ***, p < 0.001; **, p < 0.01; *p < 0.05. Data are representative from at least 2–3 experiments with similar results