Fig. 7

The tumor suppressor role of LHPP and NKX3–1 in PCa. a, b The expression pattern of LHPP in total or in separate Gleason score of 498 PCa tissues (52 normal controls) were plotted with TCGA database. Student’s t test was used for the statistical analysis of two groups comparison. One-way ANOVA test with Bonferroni’s correction was used for statistical analysis of more than two groups comparisons. c The survival probability of LHPP was determined with Kaplan–Meier analysis and log-rank test in 497 PCa patients according to the relative expression of LHPP. d The expression of LHPP and NKX3–1 in RWPE-1 and PCa cell lines were identified by western blot. GAPDH was the internal reference. e The overexpression efficiency of pLHPP plasmid (transient transfection with FuGENE HD Transfection Reagent, 0.5 μg/ml) was validated by western blot assay. GAPDH was the internal reference. f and g The colony formation assay (representative wells were presented) was used to evaluate the colony rate after overexpressing LHPP. Mann-Whitney test was used for the statistical analysis. h Flow cytometry assay (representative images were presented) and western blot assay were used to evaluate the apoptosis analysis induced by overexpressing LHPP. GAPDH was the internal reference. i and j Trans-well assay and wound-healing assay (representative wells were presented) were used to determine the cell migration after overexpressing LHPP. Mann-Whitney test was used for the statistical analysis. k The AKT phosphorylation inhibited by LHPP was analyzed by western blot assay. GAPDH was the internal reference. l All the findings in this study is concluded as a schematic diagram. Error bars represent the SD obtained from at least three independent experiments; *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001