Fig. 1

The validation, expression and stability analysis of hsa_circ_0004872 in GC. a Schematic diagram exhibits the formation of hsa_circ_0004872 (circ_4872) via the circularization of the exons 2, 3 and 4 from MAPK1 in Chr22 (red arrow). b Sanger sequencing of the RT-PCR products of hsa_circ_0004872. The black arrow indicated the splicing site of hsa_circ_0004872. c cDNA and gDNA of BGC-823 cells were used as the templates to amplify hsa_circ_0004872 and β₂-M with divergent primers and convergent primers, respectively. The results showed that hsa_circ_0004872 was amplified by divergent primers in cDNA but not gDNA. The nagative control β2-M can not be amplified by divergent primers in both cDNA and gDNA. d qRT-PCR analysis of hsa_circ_0004872 expression in GC tissues and corresponding nontumor tissues (n = 76, p < 0.0001, Student’s t-tests). e and f The RNA levels of hsa_circ_0004872 and MAPK1 in both BGC-823 and SGC-7901 cells were detected by qRT-PCR (e) or RT-PCR (f) in the presence or absence of RNase R. g Northern blot analysis of the RNA level of hsa_circ_0004872 in the GC cells treated with RNase R. h qRT-PCR analysis of the hsa_circ_0004872 and MAPK1 expression in the GC cells under the treatment with actinomycin D. All datas were the means ± SD