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Fig. 4 | Molecular Cancer

Fig. 4

From: CircECE1 activates energy metabolism in osteosarcoma by stabilizing c-Myc

Fig. 4

CircECE1 is a positive regulator of glucose metabolism in osteosarcoma. a. Schematic representation of glucose metabolism in cancer cells. b-c. OS cells infected with either control shRNA or CircECE1 shRNA were subjected to various analyses to measure the expression levels of genes involved in glucose metabolism by real-time PCR (b) or WB (c). OS cells infected with either control vector or CircECE1 overexpression vector were subjected to various analyses to measure the expression levels of genes involved in glucose metabolism by real-time PCR (b) or WB (c). d. CircECE1 overexpression increased ATP production and CircECE1 knockdown decreased ATP production. e. Mitochondrial potential increased in the presence of CircECE1 overexpression and decreased in the presence of CircECE1 knockdown. f. CircECE1 induced lactate production as indicated by the color of the medium. g. CircECE1 downregulation inhibited glucose uptake and lactate production in osteosarcoma cells. h. Extracellular acidification rate (ECAR), an indicator of glycolysis, was reduced in response to CircECE1 knockdown. Oxygen consumption rate (OCR), which reflects mitochondrial respiration, was decreased in CircECE1 inhibited osteosarcoma cells

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