Fig. 4

circCUL2, miR-142-3p and ROCK2 expression in vivo. a: FISH for circCUL2 and miR-142-3p analysis in GC and normal tissues. b and c: The miRNA levels of miR-142-3p b and mRNA level of ROCK2 c in GC tissues detected by qRT-PCR. d: The protein level of ROCK2 in GC tissues detected by western blot. e: The positive correlation of circCUL2 expression with ROCK2 expression (P < 0.01). f-g: The negative correlation of miR-142-3p expression with circCUL2 expression (P < 0.01) f and ROCK2 expression (P < 0.01) g. h: Nude mice were subcutaneously injected with SGC-7901 cells overexpressing circCUL2. i: Tumor volumes were measured in mice that received subcutaneous injections. j: After the mice were sacrificed, tumors were weighed separately. k: FISH analysis of circCUL2 and miR-142-3p in mouse tumor tissues