Fig. 5

circCUL2 regulates malignant transformation through miR-142-3p/ROCK2 in vitro. a: The mutant and putative binding site of miR-142-3p with circCUL2 (upper) and ROCK2 (lower). b: A luciferase reporter assay was used to detect the binding of miR-142-3p to circCUL2 and ROCK2 in AGS cell lines. c: qRT-PCR of circCUL2 and miR-142-3p expression in AGS cell lysates pulled down and enriched with a circCUL2-specific probe. d: RIP with an anti-AGO2 antibody in AGS and SGC-7901 cell lines was used to detect the circCUL2 and miR-142-3p mRNA levels (right). The AGO2 protein level was determined by western blotting (left). e and f: Cotransfection of miR-142-3p mimics and circCUL2 overexpression plasmid or miR-142-3p inhibitor and circCUL2-specific siRNA to detect the mRNA e and protein f levels of ROCK2 in AGS cell lines. g-k: Cotransfection of miR-142-3p mimics and circCUL2 overexpression plasmid to investigate malignant transformation of cells with CCK-8 g, EdU h, colony formation i, wound healing j and Transwell k assays in the AGS cell line