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Fig. 7 | Molecular Cancer

Fig. 7

From: circCUL2 regulates gastric cancer malignant transformation and cisplatin resistance by modulating autophagy activation via miR-142-3p/ROCK2

Fig. 7

circCUL2 activates autophagy by targeting miR-142-3p in cisplatin-resistant GC cells. Cisplatin-resistant AGS and SGC-7901 cells (AGS/DDP and SGC-7901/DDP) were treated with the following conditions: mock, DDP+ vector, DDP+ circCUL2 overexpression plasmid, DDP+ circCUL2+ miR-NC, and DDP+ circCUL2+ miR-142-3p mimics. a: Western blot analysis was used to detect ROCK2 and autophagy-related proteins (P62, Beclin 1 and LC3 A/B). b: Cells were transfected with the GFP-LC3B plasmid for 24 h to detect autophagy activation. c: Immunofluorescence (IF) was used to detect the activation of autophagy in all cells. d: Electron microscopy was used to view the autophagosome number in cisplatin-resistant cells

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