Fig. 1

The PVT1 locus has an enhancer element that targets MYC. a Enhancer activity within chromosome 8, and specifically within the 8q24 region, among six different cancer types (Colorectal cancer: Gastric cancer, Breast cancer, Lung cancer, Prostate cancer, and Melanoma) according to the FANTOM5 database. b Comparison panel of two different analyses; H3K27ac signal in a cell line panel (GM12878, H1-hESC, HSMM, HUVEC, K562, NHEK, NHLF) and in HCT116 (CRC cell line); Chia-PET in K562 leukemia cell line (as revealed by exploring the UCSC genome browser). c 3C assay to detect the PVT1-MYC interaction in HCT116 cells. d RT-qPCR determination of the PVT1 lncRNA, U6 snRNA (nucleus-specific), and GAPDH mRNA (cytoplasm-specific) levels in nuclear and cytosolic extracts from HCT116 cells. e Plot for the PVT1 lncRNA and MYC mRNA expression levels in CRC. f Effect of knockdown of the PVT1 lncRNA on expression levels of the PVT1 lncRNA and MYC mRNA in Caco-2 and HCT116 cells as determined by RT-qPCR. g Western immunoblotting to determine the effect of knockdown of the PVT1 lncRNA on MYC protein levels in Caco-2 and HCT116 cells. *P < 0.05, **P < 0.01, ***P < 0.001