Fig. 5

circPARD3 promotes LSCC cell proliferation, migration, invasion and chemoresistance via inhibiting autophagy. a and b FD-LSC-1 and Tu 177 cells were transfected with siRNAs targeting circPARD3 (si-circ-1, si-circ-2) or si-NC, then cell proliferation was determined by CCK8 (a) and colony formation assays (b). c and d The migration (c) and invasion (d) abilities of FD-LSC-1 and Tu 177 cells after silencing of circPARD3 were detected by Transwell assays. Scale bar, 200 μm. e FD-LSC-1 and Tu 177 cells were transfected with siRNAs targeting circPARD3 or si-NC for 24 h, and then treated with various concentration of Cisplatin for another 24 h. Cell viability was detected by CCK8 assays. f FD-LSC-1 and Tu 177 cells were transfected with si-circPARD3 (si-circ) or si-NC for 24 h, and then treated with 3-MA (2.5 mM). Cell proliferation was determined by CCK8 assays. g-i FD-LSC-1 and Tu 177 cells were transfected with si-circPARD3 or si-NC for 24 h, then treated with 3-MA (2.5 mM) for additional 24 h. The migration (g) and invasion (h) abilities of FD-LSC-1 and Tu 177 cells after various treatments were detected by Transwell assays. Scale bar, 200 μm. Cell viability of LSCC cells treated with various concentrations of Cisplatin for 24 h was determined by CCK8 assays (i). Error bars represent SD of three independent experiments. *P < 0.05, **P < 0.01