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Fig. 7 | Molecular Cancer

Fig. 7

From: circPARD3 drives malignant progression and chemoresistance of laryngeal squamous cell carcinoma by inhibiting autophagy through the PRKCI-Akt-mTOR pathway

Fig. 7

PRKCI is a direct downstream target gene of miR-145-5p in LSCC cells. a Venn analysis of mRNAs downregulated in Tu 177 cells overexpressing miR-145-5p (Dataset 1), bioinformatics predicted miR-145-5p target genes (Dataset 2), and mRNAs upregulated in 107 LSCC tissues from RNA sequencing data (Dataset 3). b and c FD-LSC-1 and Tu 177 cells were transfected with miR-145-5p mimics or NC mimics for 48 h, then the expression of PRKCI was detected by qPCR analysis (b) and western blotting (c). d Luciferase reporter assay of FD-LSC-1 cells cotransfected with miR-145-5p mimics and luciferase reporter plasmid containing wild-type (WT) and miR-145-5p binding site mutated (Mut) PRKCI 3′ UTR sequence. N.S., no significant. e Expression of PRKCI in FD-LSC-1 and Tu 177 cells silencing or overexpression of PRKCI was verified by western blotting. f Cell proliferation of LSCC cells silencing of PRKCI was determined by colony formation assays. g and h The migration (g) and invasion (h) abilities of FD-LSC-1 and Tu 177 cells after PRKCI knockdown were evaluated by Transwell assays. i Cells were transfected with si-PRKCI or si-NC for 24 h, then treated with various concentrations of Cisplatin for 24 h. Cell viability was determined by CCK8 assays. j FD-LSC-1 and Tu 177 cells were transfected with miR-145-5p mimics, or transfected with miR-145-5p mimics and infected with PRKCI overexpression lentiviruses simultaneously. Cell proliferation was determined by CCK8 assays. k and l The migration (k) and invasion (l) abilities of FD-LSC-1 and Tu 177 cells overexpressing PRKCI and miR-145-5p were detected by Transwell assays. Scale bar, 200 μm. m FD-LSC-1 and Tu 177 cells overexpressing PRKCI were transfected with miR-145-5p mimics for 24 h, then treated with various concentrations of Cisplatin for 24 h. Cell viability was determined by CCK8 assays. Error bars represent SD of three independent experiments. **P < 0.01

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