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Fig. 1 | Molecular Cancer

Fig. 1

From: CSN8 is a key regulator in hypoxia-induced epithelial–mesenchymal transition and dormancy of colorectal cancer cells

Fig. 1

CSN8 expression is upregulated in CRC tissues, and is involved in the EMT and dormancy of CRC cells. a Tissue microarray assays analyzed the expression of CSN8 in CRC tissues. Representative immunohistochemistry images showed the expression and subcellular distribution of CSN8 in tumor tissues or tumor-adjacent tissues. b Nuclear CSN8 expression is higher in tumor tissues than in tumor-adjacent tissues. c Kaplan–Meier survival analysis was conducted according to the CSN8 levels in CRC patients (log-rank test). d Representative immunohistochemistry images show the expression of CSN8 and E-Cadherin in tumor tissues. e Western-blot analysis of protein levels of E-Cadherin, N-Cadherin, and Slug in CSN8-overexpressed CRC cells and control cells. f, g Representative scratch-wound images showing the healing ability of CSN8-overexpressed CRC cells and control cells. h, i The migration ability of CSN8-overexpressed CRC cells and control cells was determined by Transwell migration assay. j, k The invasive ability of CSN8-overexpressed CRC cells and control cells was analyzed by Matrigel invasion assay. l Real-time PCR was used to analyze the mRNA expression of EMT-associated genes. m CCK-8 assay analyzed the proliferation activity of CSN8-overexpressed CRC cells and control cells. np Trypan blue exclusion assay was used to analyze the cell viability of CSN8-overexpressed CRC cells and control cells cultured under 20% O2 or 1% O2, serum deprivation, or 5-FU (20 μg/mL) conditions. q Western blot analysis of the protein levels of dormancy-associated genes. r Real-time PCR was used to analyze the mRNA expression of dormancy-associated genes. s Human CRC tissue cDNA arrays showing changes in CSN8, NR2F1, or HIF-1α transcript expression. Data are presented as the mean ± standard deviation.*P < 0.05; **P < 0.01; ns, P ≥ 0.05

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