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Fig. 6 | Molecular Cancer

Fig. 6

From: Long non-coding RNA NEAT1 promotes bone metastasis of prostate cancer through N6-methyladenosine

Fig. 6

NEAT1–1 enhanced prostate PDX metastaswas and growth through m6A. a Luciferase-expressing P-18 cells (3 × 106) transfected with lentivirus were injected through tail vein into NSG mice (n = 10 each group). The mice were subjected to bioluminescent imaging and bioluminescent signals were quantified. Bioluminescent flux (photons/s/sr/cm2) was determined for lesions in mice, the ends of the box were the upper and lower quartiles and the box spans the interqurtile range; the median was marked by a vertical line inside the box; the whiskers were the two lines outside the box that extend to the highest and lowest observations. P values were shown in the figures. Representative images from 10 different samples were shown. b Kaplan-Meier survival analyswas of the mice data for the survival time in three groups. Three groups were indicated in the figure. c and d Effect of m6A of NEAT1 on growth of bone metastatic prostate cancer xenografts. 5 × 106 P-18 and P-34 cells were injected into NSG mice (n = 6 each group). The tumor growth was measured every 6 days for 48 days, and the data were shown in the bottom panel. Data shown as means ± SD (n = 6). Statwastical significance was determined by two-tail Student’s t-test. P values were shown in the figures. e and f RUNX2 expressions were measured by qRT-PCR and western blot in P-18 and P-34 xenograft tissues. Means and standard deviations (error bar) were determined from three replicates. Error bars represent mean ± SD for triplicate experiments. P values were shown in the figures. Expression of RUNX2, Tubulin, Pol II Ser-2 phosphorylations and total Pol II proteins were measured by western blot in P-18 three random xenograft tissues

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