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Fig. 2 | Molecular Cancer

Fig. 2

From: Hypoxia downregulated miR-4521 suppresses gastric carcinoma progression through regulation of IGF2 and FOXM1

Fig. 2

miR-4521 inhibits invasion and metastasis in GC. a-b The invasive and migratory abilities of SGC7901-H cells stably expressing miR-4521, SGC7901-L cells stably silencing miR-4521 and the respective control cells were analyzed by transwell and wound healing assays. Scale bars, 150 μm. Error bars, SD from three independent experiments performed in triplicate. *P < 0.05, **P < 0.01. c-d Nude mice were intraperitoneally injected with 5 × 105 SGC7901-H and SGC7901-L cells stably expressing the indicated constructs. After 21 days, the metastasis signal was measured by BLI, the mice were sacrificed, and tumor nodules in the abdominal cavity were examined. Representative images of bright view and bioluminescent view are shown. Arrows or circles indicate metastatic nodules. The number of metastatic nodules was quantified. Error bars, SD (n = 5 mice/group). *P < 0.05, **P < 0.01. e-f SGC7901-H cells with stable miR-4521 overexpression or controls (1 × 106 cells) were injected into the nude mice via the tail vein, with 5 mice per group. Representative images of the luciferase signals of lung metastasis and bright view and H&E staining of lung tissue are shown. The number of metastatic foci was quantified. Scale bars, 100 μm. Error bars indicate SD. **P < 0.01

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