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Fig. 5 | Molecular Cancer

Fig. 5

From: Hypoxia downregulated miR-4521 suppresses gastric carcinoma progression through regulation of IGF2 and FOXM1

Fig. 5

miR-4521 inactivates the AKT/GSK3β/Snai1 pathway. a Western blot analysis of the protein expression in miR-4521-overexpressing cells, miR-4521-knockdown cells and control cells. b Immunohistochemistry analysis of E-cadherin and Vimentin in mouse lung tumors generated by miR-4521-overexpressing SGC7901-H cells and the control cells. Scale bar, 100 μm. c Analysis of the protein expression in the indicated cells in hypoxia or normoxia. d miR-4521-expressing cells were transfected with a plasmid encoding FOXM1 or treated with 100 ng/ml IGF2 for 24 h, while miR-4521-silenced cells were transfected with FOXM1 shRNA or treated with PPP (10 nM) for 24 h. Western blotting was performed to detect protein expression. e miR-4521-silenced cells were treated with the AKT inhibitor MK2206 (0.5 μM) for 24 h, and the protein expression was measured by Western blotting. f Cell invasion ability of miR-4521-silenced cells that were treated with AKT inhibitor MK2206 (0.5 μM) for 48 h. Scale bar, 150 μm. Experiments were repeated three times, and error bars represent SD. *P < 0.05; **P < 0.01

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