Fig. 3

SRSF6 promotes autophagy activity through regulating alterative splicing of PICLAM. a Schematic diagram of PICALM splice variants. b RIP and RT-PCR for detecting the interaction between SRSF6 protein and PICALM mRNA (upper panels: RT-PCR for PICALM mRNA; lower panels: immunoblotting for SRSF6-flag protein). c Depletion of SRSF6 leads to a shift of PICALM splicing module in MGC803 cells. d The interference and overexpression of PICLAML and the overexpression efficiency of PICALMS were assessed by western blot. e CCK8 assays were used to evaluate the interference and overexpression of PICLAML and overexpression of PICALMS on the sensitivity of MGC803 cells to oxaliplatin and 5-FU. f The effect of PICALML and PICALMS on the expression of LC3II in MGC803 cells in the presence of CQ was detected by western blot. g PICLAML or PICLAMS were re-introduced into MGC803/shSRSF6 cells for drug sensitivity test. (H) PICLAML and PICLAMS were re-introduced into MGC803/shSRSF6 cells for western blot to detect the expression level of LC3II. Representative figures are shown. The results are from three independent experiments. One-way ANOVA; mean ± SD; the asterisk (**) indicates P < 0.01