Fig. 2

Targeting CDK7 protects against the LPS-induced inflammatory response. a Schematic of the murine CRS model triggered by LPS. LPS was intraperitoneally injected following THZ1 pretreatment. b Survival of mice in 3 independent experiments (n = 16). A log-rank Mantel-Cox was performed for statistical analysis. c Serum levels of murine cytokines from mice at 12 h following LPS stimulation. d Tissue sections were obtained from mice after THZ1 pretreatment and LPS stimulation and stained with H&E. e Representative plots and percentages of living CD11b+CD86+ myeloid cells in peritoneal lavage. f Representative plots and percentages of living F4/80+ macrophages, Ly6C+ monocytes and F4/80-Ly6C- (double negative, DN) myeloid cells in peritoneal lavage. g Transcriptional levels of IL1B, TNFA, STAT1 in sorted peritoneal macrophages from mice pretreated with THZ1 at 12 h following LPS stimulation. Data are the mean ± SD, n = 3 or 5 in c to g. ***P < 0.001, **P < 0.01, and *P < 0.05 by one-way ANOVA in c and e and unpaired t test in d and g