Fig. 4

Landscape of super enhancers in macrophages. a Venn diagram depicting the overlap between SEs in control mTHP-1 or in LPS-stimulated mTHP-1 pretreated with THZ1 or not. In total, 869 SEs were associated with 1280 genes and 58 genes among them were sensitive to THZ1 pretreatment. b Metagene representations of the mean H3K27ac ChIP-seq density for H3K27ac across typical enhancers and SE domains. Metagenes were centered on the enhancer regions, and the length of the enhancer reflected the difference in median lengths of super and typical enhancers. An additional 2 kb surrounding each enhancer region is also shown. c Lists of representative SE-associated genes that were sensitive to THZ1. These genes were divided into 3 categories according to their functions. d Total H3K27ac signal in enhancer regions for all stitched enhancers in control mTHP-1, LPS-stimulated mTHP-1 pretreated with THZ1 or not. Enhancers were ranked by increasing H3K27ac ChIP-seq signal. SEs of interest are shown along with their respective ranks and their associated genes. e ChIP-seq profiles for H3K27ac at the representative SE-associated gene loci in mTHP-1 cells. f STAT1 and STAT4 binding motifs enriched at constituent enhancers within SEs and corresponding P values. g THZ1-sensitive SEs containing STAT1 binding motifs were enriched using gene set enrichment analysis