Table 2 AURKA downstream substrates

YBX1

T62, S102

Consensus motif, in vitro kinase assay, autoradiography

Increases YBX1 protein stability, regulate EMT, CSC and chemoresistance.

[17]

LDHB

S162

In vitro kinase assay, MS analysis, point mutation, anti-phosphoserine

Promotes glycolysis and biosynthesis; Promotes tumor growth.

[45]

Merlin

S518

In vitro kinase assay, specifc antibody

Weakens merlin interaction with tubulin.

[46]

RPS6KB1

T389

P-RPS6KB1 (T389) specifc antibody

Reduces survival of KRAS-mutant gastrointestinal cancer cells

[47]

CENP-A

S7

In vitro kinase assay, truncation mutants, autoradiography

Regulates mitosis especially kinetochore function.

[48]

LKB1

S299

In vitro kinase assay, MS analysis,

point mutation, autoradiography

Impairs LKB1 interaction with and phosphorylation of AMPK.

[49]

KCTD12

S243

Point mutation, IP, anti-phosphoserine

Promotion of cancer cell proliferation and tumorigenesis.

[26]

CHIP

S273

Point mutation, in vitro kinase assay, autoradiography

Promotes AR degradation via the proteasome pathway.

[50]

ALDH1A1

T267, T442, T493

Consensus motif, point mutation, in vitro kinase assay, autoradiography

Regulates protein stability, EMT and CSC phenotypes.

[16]

Twist

S123, T148,

S184

Consensus motif, point mutation, in vitro kinase assay, autoradiography

Regulates protein stability, subcellular localization, EMT, the CSC phenotype and drug resistance.

[15]

YAP

S397

In vitro kinase assay, truncation mutants, MS, consensus motif

Enhances transforming ability.

[51]

TACC3

S558

MS, point mutation, in vitro kinase assay, autoradiography

Regulates TACC3 localization to centrosomes and proximal mitotic spindles.

[52]

HDM2

S166

In vitro kinase assay, specifc antibody

NA

[53]

β-catenin

S552,

S675

In vitro kinase assay, truncation mutants, autoradiography

Increases its stability and transcriptional activity

[54]

ERα

S167,

S305

In vitro kinase assay, truncation mutants, autoradiography

Leading to increase in ERα DNA-binding and transcriptional activity.

[55]

BimEL

S93/94/98

Consensus motif, in vitro kinase assay, specifc antibody

NA

[56]

GSK-3β

S9

In vitro kinase assay, specifc antibody

Leading to accumulation and activation of the β-catenin/TCF complex.

[57]

PLK1

T210

In vitro kinase assay, specifc antibody

Centrosomal organization.

[58]

IκBα

S32, S36

Specifc antibody

NFκB activation.

[59]

VHL

S72

In vitro kinase assay, MS, autoradiography

NA

[60]

PHLDA1

S98

In vitro kinase assay, consensus motif, autoradiography

Negatively regulates PHLDA1 by promoting PHLDA1 degradation.

[40]

Histone H3

S10

In vitro kinase assay, autoradiography

Involved in the initiation of mitosis.

[61]

YY1

S365

In vitro kinase assay, truncation mutants, consensus motif, autoradiography

Abolishes its DNA binding activity and transcriptional activity.

[62]

SDCBP

S131,

T200

In vitro kinase assay, consensus motif, autoradiography, anti-phospho-serine/threonine

Stabilizes SDCBP protein and regulates its oncogenic function.

[63]

SOX2

S220, S251

In vitro kinase assay, MS

Maintains the ratio of stem-cell like cells.

[64]

CPAP

S467

In vitro kinase assay, truncation mutants, MS, autoradiography

Is required for the integrity of the spindle pole during mitosis.

[65]

RalA

S194

In vitro kinase assay, consensus motif, autoradiography

Enhances cell migration and anchorage-independent growth.

[66]

CDC25B

S353

MS, in vitro kinase assay, specifc antibody

Control of the onset of mitosis.

[67]

NDEL1

S251

MS, in vitro kinase assay, autoradiography

Centrosomal maturation, separation, and TACC3 recruitment.

[68]

ASAP

S625

MS, in vitro kinase assay, autoradiography

Bipolar spindle assembly.

[69]

MBD3

S24

In vitro kinase assay, autoradiography, consensus motif

NA

[70]

RASSF1A

T202, S203

Consensus motif, in vitro kinase assay, autoradiography

Induces M-phase cell cycle arrest.

[71]

P53

S215,

S315

S106

Consensus motif, in vitro kinase assay, autoradiography

S215: Abrogates DNA binding and transactivation activity;

S315: Inactivates p53 by enhancing its proteolytic degradation.

S106: Inhibit the interaction of p53 with MDM2 and prolong the half-life of p53.

[72] [73]

[74]

HURP

S627, S725,

S757, S830

In vitro kinase assay,autoradiography, MS

Regulates HURP stability and exhibits serum-independent growth.

[75]

PP1

NA

In vitro kinase assay,autoradiography

Inhibits PP1 activity at mitosis.

[43]

P73

S235

Consensus motif, in vitro kinase assay, autoradiography

Abrogates its transactivation function; Regulates subcellular localization.

[76]

TPX2

S121,

S125

Consensus motif, in vitro kinase assay, autoradiography

Maintains metaphase spindle length by regulating the microtubules flux.

[77]

Lats2

S83

In vitro kinase assay, truncation mutants, autoradiography

Regulates centrosomal localization.

[78]

LIMK2

S283, T494,

T505

Consensus motif, in vitro kinase assay, autoradiography

Inhibits LIMK2 degradation and increases LIMK2 kinase activity.

[14]