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Fig. 2 | Molecular Cancer

Fig. 2

From: ITGBL1 is a new immunomodulator that favors development of melanoma tumors by inhibiting natural killer cells cytotoxicity

Fig. 2

ITGBL1 modulates tumor growth by inhibiting natural killer. a 0.15 × 106 B16F10 cells overexpressing or not ITGBL1 were injected subcutaneously in C57BL/6 J, and tumor weight was monitored after 12 days (mean tumor weight in g ± s.e.m.). Picture of representative tumors at 12 days is shown on the top (n = 6 mice, *P < 0.05). B) mRNA form tumor overexpressing ITGBL1 (ITGBL1) or not (Ctl) were quantified for IFNγ and GZMB by QPCR and expressed as relative quantification in fold change. b 0.15 × 106 B16F10 cells overexpressing or not ITGBL1 were injected subcutaneously in Nude mice and tumor volume was monitored for 15 days (mean tumor volume in g ± s.e.m.). Picture of representative tumors at 15 days is shown on the top (n = 6 mice, *P < 0.05). c 0.15 × 106 BP cells overexpressing (ITGBL1) or not ITGBL1(Ctl) were injected subcutaneously in C57BL/6 J. When tumor reached 50mm3, mice were treated with anti-PD1 (PD1) and tumor growth was monitored. Tumor volume is displayed (mean tumor volume in mm3 ± s.e.m.). (n = 6 mice, *P < 0.05). d 0.15 × 106 BP cells overexpressing or not ITGBL1 were injected subcutaneously in NSG, and tumor growth was monitored for 14 days (mean tumor volume in mm3 ± s.e.m.). Picture of representative tumors at 14 days is shown on the top (n = 6 mice). e NK-92 cells were activated (PMA/iono) for 1 h in presence or absence of ITGBL1 (10 ng/ml). mRNA was extracted and analyzed by QPCR for IFNγ expression. Results shown are mean+/−SD of 3 independent experiments (left panel). IFNγ secretion in activated NK-92 cells incubated for 6 h with or without ITGBL1. Results shown are mean+/−SD (right panel). f Red stained Skmel-28 cells, overexpressing ITGBL1 or not, were cocultured with NK at ratio 1 NK/1 melanoma and treated with anti-PD1 (5 μg/ml) or its isotype control for 24 h. Cells were analyzed by FACS with dapi and % of melanoma positive for dapi was quantified and expressed as % of melanoma death (mean+/−SD, n = 3, *P < 0.05, **P < 0.001)

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