Fig. 2

JPX overexpression promoted cell growth and proliferation in vitro and in vivo. The CCK-8 assays were performed to determine the viability of lung cancer cells treated with si-JPX#1 (a) or pcDNA3.1-JPX (b) in SPC-A1 and NCI-H1299 cells. Colony formation assays were used to detect the proliferation of lung cancer cells after transfection with si-JPX#1 (c) or pcDNA3.1-JPX (d) in SPC-A1 and NCI-H1299 cells. The bar charts represent the numbers of cell colonies. (e) NCI-H1299 cells with empty vector were injected into the upper nude mice (n = 7), while NCI-H1299 cells stably expressing JPX were injected into the lower nude mice (n = 7). Representative images of xenograft tumors are shown in the right panel. (f, g) Tumor volume and weight in the xenograft mice from the JPX overexpression group and the control group. Data are shown as the mean ± SD in three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001