Fig. 4

JPX targeted the miR-33a-5p in lung cancer cells as a ceRNA. (a) The subcellular position of JPX in the cytoplasm or nucleus. GAPDH and U6 were used as the cytoplasmic and nuclear control, respectively. (b)The potential binding sites of miR-33a-5p and JPX. (c) Relative luciferase activities of wild type (WT) and mutated (MUT) JPX reporter plasmid in SPC-A-1 and NCI-H1299 cells co-transfected with miR-33a-5p mimics. (d) The RT-qPCR assays were utilized to measure miR-33a-5p expression in 65 lung cancer tissues and paired normal tissues. (e) Pearson’s correlation analysis determined the relationship between JPX and miR-33a-5p expression in 20 lung cancer tissues. (f) Relative expression of miR-33a-5p in lung cancer cell lines compared with that in the normal epithelial cell line. (g) Relative miR-33a-5p expression after JPX knockdown. (h) Detection of JPX expression by RT-qPCR after overexpression of miR-33a-5p. Data are shown as the mean ± SD based on three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001