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Fig. 8 | Molecular Cancer

Fig. 8

From: lncRNA JPX/miR-33a-5p/Twist1 axis regulates tumorigenesis and metastasis of lung cancer by activating Wnt/β-catenin signaling

Fig. 8

JPX/miR-33a-5p/Twist1 promoted EMT progression in lung cancer cells by activating the Wnt/β-catenin pathway. Immunoblot assay of E-cadherin, N-cadherin, Vimentin, Twist1, GSK-3β and β-catenin proteins in SPC-A-1 and NCI-H1299 cells transfected with si-JPX#1 (a), pcDNA3.1-JPX (b), and miR-33a-5p + pcDNA3.1-JPX (c). Western blot analysis for β-catenin, E-cadherin, and N-cadherin in SPC-A-1 and NCI-H1299 cells after transfection with pcDNA3.1-JPX (d), and si-CTNNB1+ pcDNA3.1-JPX (e, f). Western blotting was conducted to verify the protein expression of GSK-3β and β-catenin in SPC-A-1 and NCI-H1299 cells transfected with si-Twist1#2/#4 (g) and si-Twist1#2/#4 + pcDNA3.1-JPX (h, i). The numbers indicate the quantification of relative protein amount. GAPDH was used as an internal control. (j) Schematic diagram of the regulatory mechanism of the JPX/miR-33a-5p/Twist1 axis in promoting lung cancer cells proliferation and metastasis. Twist1 could participate in the EMT process through the Wnt/β-catenin pathway, ultimately affecting the proliferation and metastasis of lung cancer cells, which could be inhibited by miR-33a-5p and enhanced by JPX as a ceRNA. Data are shown as the mean ± SD based on three independent experiments

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