Fig. 2

The proliferation and migration of CRC is promoted by circ_001680. a Relative expression of circ_001680 in lentivirus-infected SW480 and HCT116 CRC cell lines. b CCK8 assay comparing the effects of circ_001680 on cell growth between the SW480-vector and SW480-circ_001680 groups (left) and between the SW480-scramble and SW480-circ_001680 inhibitor groups (right). c, d Representative images of the colony formation assay in the indicated cells; d, statistical analysis of the colony formation results. e, f E, Representative transwell results comparing the effects of circ_001680 on cell migration between the SW480-vector and SW480-circ_001680 groups (left) and between the SW480-scramble and SW480-circ_001680 inhibitor groups (right); f, statistical analysis of the transwell assay results. g, h Wound healing assay results showing the differences in migration capacities in the indicated cells at 4 regular intervals (left); statistical analysis of the wound healing assay results (right). i Image of the tumor xenograft model (upper); differences of tumor volume between the SW480-vector and SW480-circ_001680 cells, which were injected into subcutaneous nude mice (lower). j Results of hematoxylin and eosin (H&E) and Ki-67 immunohistochemistry analysis of tumors generated using the tumor xenograft model (left); statistical analysis of Ki-67 positive cells (right). The error bars represent the means±SDs from three independent experiments. **p < 0.01, ***p < 0.005, ****p < 0.001