Skip to main content
Fig. 5 | Molecular Cancer

Fig. 5

From: Hsa_circ_001680 affects the proliferation and migration of CRC and mediates its chemoresistance by regulating BMI1 through miR-340

Fig. 5

Circ_001680 enhances the colorectal cancer CSC population in vitro. a The number of subpopulations with the CD44+/CD133+ phenotype in the indicated SW480 (upper) and HCT116 (lower) cells. b Statistical results of the number of subpopulation cells with the CD44+/CD133+ phenotype. c The apoptosis assay conducted by flow cytometry in the indicated group. Flow cytometry analyses of SW480 cells treated with 1.0 μM doxorubicin for 24 h. d Statistical analysis of flow results. Annexin-positive/PI-negative cells were analyzed to determine the apoptosis rate. Error bars represent the mean ± SD from three independent experiments. e Typical images from the sphere formation assay among vector, circ_001680, circ_001680 inhibitor and circ_001680 + miR-340. f Graphical illustration showing the sphere formation efficiency from Figure 5C. g Western blot analysis of CD133, SOX2 and CD44 in the indicated lentivirus-infected cells. h qRT-PCR analysis of CD133, CD44 and SOX2 in the indicated cells.

Back to article page

Molecular Cancer

ISSN: 1476-4598