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Fig. 6 | Molecular Cancer

Fig. 6

From: Hsa_circ_001680 affects the proliferation and migration of CRC and mediates its chemoresistance by regulating BMI1 through miR-340

Fig. 6

Overexpression of circ_001680 suppresses the sensitivity of CRC cells to irinotecan. a The numbers of subpopulation cells with the CD44+/CD133+ phenotype in the indicated SW480 (upper) and HCT116 (lower) cells. b Quantification of cells with the CD44+/CD133+ phenotype is shown in the histogram (*P < 0.05, **P < 0.01). c Typical images from the sphere formation assay in the indicated lentivirus-infected cells treated with or without irinotecan. d Bar graph showing the sphere formation efficiency (*P < 0.05, **P < 0.01). e CCK8 analyzed cell growth capacity in the indicated cells (**P < 0.01). f Apoptosis assay of the indicated cells by flow cytometry (middle). Statistical analysis of the flow cytometry results (right). g Western blot analysis of CD44, CD133, BMI1 and SOX2 in the indicated cells. h qRT-PCR analysis of BMI1, CD44, CD133 and SOX2 in the indicated cells. i Images of the tumor xenograft model (n = 5). j Tumor volume statistics among the four indicated groups on the indicated days. k The tumor weight statistics among the four indicated groups. l H&E and immunohistochemistry analyses of xenograft tumors. IHC staining was performed using antibodies against caspase-3, BMI1, CD44 and SOX-2. The error bars represent the means±SDs from three independent experiments (*P < 0.05, **P < 0.01)

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