Fig. 3

SNHG12 overexpression confers TMZ resistance. a qRT-PCR analysis of SNHG12 expression in Pri GBM and N3S cells transfected with empty vector or pcDNA-SNHG12. b Western blot analysis of caspase-3 and PARP in TMZ-sensitive cells with or without TMZ treatment (200 μM, 48 h). β-actin was used as the loading control. c Flow cytometric analysis revealing the effect of SNHG12 overexpression on the apoptosis of TMZ-sensitive cells with or without TMZ treatment (200 μM, 48 h). d TUNEL analysis of SNHG12 overexpression cells or empty vector with or without TMZ treatment (200 μM, 48 h). Scale bar = 50 μm. e Colony formation assays of SNHG12 overexpression or empty vector TMZ-sensitive cells with or without TMZ treatment (200 μM, 48 h). f Overexpression of SNHG12 increased the levels of p-Rb, cyclin D1 but not CDK4 and CDK6 in TMZ-sensitive cells. g The cell cycle distribution was analyzed by flow cytometric analysis in TMZ-sensitive cells transfected with empty vector or pcDNA-SNHG12. Data are presented as the mean ± SEM from three independent experiments. Significant results were presented as NS non-significant, *P<0.05, **P<0.01