Skip to main content
Fig. 7 | Molecular Cancer

Fig. 7

From: DNA-methylation-mediated activating of lncRNA SNHG12 promotes temozolomide resistance in glioblastoma

Fig. 7

The effects of MAPK1 and E2F7 on GBM cell proliferation and survival. a CCK-8 assay analysis of the effect of MAPK1 or E2F7 overexpression on Rec GBM cells after knocking down SNHG12 upon TMZ treatment at the indicated concentrations for 48 h. b Colony formation analysis of Rec GBM and N3T3rd cells after co-transfected with si-ctrl, si-MAPK1 or si-E2F7. c Flow cytometric analysis of TMZ-induced (200 μM, 48 h) apoptosis in Rec GBM and N3T3rd cells transfected with si-ctrl or si-MAPK1. d The cell cycle distribution was analyzed by a flow cytometer in Rec GBM and N3T3rd cells. e Transfection of sh-SNHG12 or pcDNA-MAPK1 into Rec GBM and N3T3rd cells affected expression of MAPK signaling pathway-associated protein. Expression of the indicated proteins was detected by western blot. f Rescue assays for immunoblotting analysis of MAPK signaling pathway-associated protein after transfection of pcDNA-SNHG12 or si-MAPK1 into Pri GBM and N3S cells. g Western blot analysis of indicated proteins in Rec GBM cells and N3T3rd cells transfected with sh-SNHG12–2 and miR-129-5p inhibitor. h Western blot analysis of indicated proteins in Pri GBM cells and N3S cells transfected with pcDNA-SNHG12 and miR-129-5p mimics. Data are presented as the mean ± SEM from three independent experiments. Significant results were presented as NS non-significant, **P<0.01, ***P<0.001

Back to article page