Fig. 5

CircPTK2 targeted vimentin protein to regulate growth and metastasis of CRC. a The correlation between the expression level of circPTK2 and vimentin. b Western blot assay showing the protein vimentin pulled down by biotin-labeled circular RNA probes from the lysates of SW620 and LOVO cells. c Mass spectrometry assays revealing the protein vimentin pulled down by biotin-labeled circPTK2 from the lysates of SW620 and LOVO cells. d Western blot assay showing circPTK2 overexpression up-regulated vimentin, MMP2/9 and CXCR4, and down-regulated E-cadherin. e Dual RNAscope and IHC assay indicating the co-localization of circPTK2 (red) and vimentin (brown) in CRC tissues. f RNA pulldown and western blotting assay showing the target sites for circPTK2 on vimentin. g-h SW480 cells were transfected with circPTK2-overexpressing plasmid as well as vimentin siRNA for 48 h. Cells were used for staining with crystal violet or injected into subcutaneous tissue of nude mice. Colony formation and tumor growth were detected. Colony formation and tumor growth of SW480 cells were shown. i-j SW620 cells were transfected with circPTK2 siRNA plasmid as well as vimentin-overexpressing plasmid for 48 h. Cells were used for staining with crystal violet or injected into subcutaneous tissue of nude mice. Colony formation and tumor growth of SW620 cells were shown. P values were calculated by t-test