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Fig. 5 | Molecular Cancer

Fig. 5

From: Long non-coding RNA SOX2OT promotes the stemness phenotype of bladder cancer cells by modulating SOX2

Fig. 5

SOX2OT positively regulates SOX2 expression by sponging miR-200c. a The RNA FISH results revealed that SOX2OT was distributed mostly in the cytoplasm of BCSCs. b SOX2OT and SOX2 have common putative binding sites within the miR-200 cluster. c: Knockdown of SOX2OT increased miR-200 s expression in BCSCs. d miR-200c expression negatively correlated with SOX2OT/SOX2 expression in bladder cancer tissues. e SOX2OT/SOX2-Wt and Agomir200c co-transfection significantly inhibited luciferase activity, and SOX2OT/SOX2-Mut and Agomir200c co-transfection failed to affect luciferase activity. f Knockdown of SOX2OT decreased the luciferase activity of cells transfected with SOX2-Wt. g Overexpressing miR-200c decreased SOX2 expression in BCSCs. h: Knockdown of miR-200c increased SOX2 expression in BCSCs transfected with sh-SOX2OT. i and j Knockdown of miR-200c significantly reversed the spheroid-formation ability inhibition induced by silencing SOX2OT.The data are shown as the mean ± SD. *p < 0.05; **p < 0.01

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