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Fig. 2 | Molecular Cancer

Fig. 2

From: RETRACTED ARTICLE: Exosome-mediated lncRNA AFAP1-AS1 promotes trastuzumab resistance through binding with AUF1 and activating ERBB2 translation

Fig. 2

Knockdown of AFAP1-AS1 reverses trastuzumab resistance of breast cancer cells. a AFAP1-AS1 was silenced by two specific small interfere oligonucleotides in SKBR-3-TR and BT474-TR cells, **P < 0.01. b-c CCK8 assay revealed that knockdown of AFAP1-AS1 promoted trastuzumab-induced cell cytotoxicity. d-e EdU staining was performed to show the effect of AFAP1-AS1 knockdown in cell proliferation, *P < 0.05. f-g Transwell migration assay was done to show the effect of AFAP1-AS1 on cell migration ability, **P < 0.01. h qPCR verified the upregulation of AFAP1-AS1 after transduction of AFAP1-AS1-loaded plasmids in SKBR-3 and BT474 cells, *** P < 0.001. i CCK8 assay showed that overexpression of AFAP1-AS1 promoted cell viability, *** P < 0.001. j-k EdU staining showed that overexpression of AFAP1-AS1 increased cell proliferation in SKBR-3 and BT474 cells, *P < 0.05. l-m Transwell assay showed that overexpression of AFAP1-AS1 promoted cell migratory ability, *** P < 0.001

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Molecular Cancer

ISSN: 1476-4598