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Fig. 5 | Molecular Cancer

Fig. 5

From: RETRACTED ARTICLE: Exosome-mediated lncRNA AFAP1-AS1 promotes trastuzumab resistance through binding with AUF1 and activating ERBB2 translation

Fig. 5

Exosome mediated transfer of lncRNAAFAP1-AS1 induces trastuzumab resistance. a Intercellular trafficking of exosomes among different cell lines by isolated exosomes labeled with PKH26 dye. b Treatment with exosomes derived from SKBR-3-TR increased AFAP1-AS1 level, however this effect was abrogated when SKBR-3-TR cells were silenced with AFAP1-AS1, *Pā€‰<ā€‰0.05. c-d CCK8 assay showed that SKBR-3-TR-derived exosomes induced trastuzumab resistance in SKBR-3 and BT474 cells, *Pā€‰<ā€‰0.05. e Nano-sight particle tracking analysis of the size distributions and number of exosomes from SKBR-3-TR cells treated with nSMase, GW4869. f-g CCK8 assay showed that incubation with exosomes from SKBR-3-TR cells treated with GW4869 failed to confer trastuzumab resistance to recipient cells. h-i CCK8 assay verified that knockdown of AFAP1-AS1(h) or HNRNPA2B1(i) inhibited the ability of co-cultured parental cells to acquire trastuzumab resistance

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