Fig. 1

METTL14 was down-regulated in human colorectal cancer.a Real-time PCR analysis of METTL14 and METTL3 expression levels in colorectal cancer and paired normal tissue. ns, not significant. **, p < 0.01. b Mega-sampler analysis of expression patterns of METTL14 and METTL3 in cancer and normal tissues from GEO dataset (GSE14333). P values were as indicated. c Representative images of immunohistochemical (IHC) staining of METTL14 in colorectal cancer and paired normal tissue. d Expression levels of METTL14 in different CRC cell lines and normal NCM460 cells. **, p < 0.01. e-f Kaplan-Meier analysis of RFS of CRC patients from our cohort (e) and GSE14333 dataset (f). g Multivariate analysis of clinical prognostic parameters for RFS of CRC patients in GSE14333 cohort. P values were as indicated. h Time-dependent ROC analysis showing the predictive ability of METTL14 of RFS of CRC patients. P values were as indicated. PCR was analyzed by paired student’s t-test (a) and nonpaired student’s t-test (d). Mega-sampler analysis of GEO data (b) was generated by R2 online tools (http://r2.amc.nl/). Difference of RFS (e and f) between two subgroups was analyzed with Log-rank test. Time-dependent ROC analysis was constructed with Cox model from ‘Survival’ R package