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Fig. 2 | Molecular Cancer

Fig. 2

From: METTL14 suppresses proliferation and metastasis of colorectal cancer by down-regulating oncogenic long non-coding RNA XIST

Fig. 2

Knockdown of METTL14 promoted proliferation and invasion of CRC cells in vitro.a Knockdown of METTL14 were confirmed by real-time PCR (upper panel) and western blot (lower panel) in HCT116 and HT29 cells. si1, siMETTL14–1. si2, siMETTL14–2. **, p < 0.01. b Cell count assay was performed to measure the proliferation of CRC cells transfected with siMETTL14 compared with those transfected with siNC. OD450 values were compared at indicated time points. *, p < 0.05, **, p < 0.01. ****, p < 0.0001. c Representative images and quantification of colony formation assay of HCT116 and HT29 cells transfected with siMETTL14 or siNC. Magnification, 200×. **, p < 0.01, ****, p < 0.0001. d Transwell invasion assay was performed to determine the effects of METTL14 on invasive ability of CRC cells. Magnification, 200×. *, p < 0.05, **, p < 0.01. All data were representative of three independent experiments and shown as mean ± SD. P values were determined by Student’s t-test

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