Fig. 5

METTL14 down-regulated XIST through m6A methylation activity. a Two CRC cell lines were transfected with METTL14-overexpression (METTL14-OE) plasmids and siWTAP as indicated. Protein band intensity of METTL14 and WTAP were quantified by western blot assay and shown as fold change. Statistical histograms were not shown. b The alteration of XIST expression in METTL14-OE and METTL14-OE + siWTAP cells was analyzed by real-time PCR. ns, not significant, **, p < 0.01. c-d The effects of WTAP and METTL14 on proliferative ability of CRC cells were measured by cell count assay (c) and colony formation assay (d). ns, not significant, *, p < 0.05, **, p < 0.01. e Quantitative analysis of transwell invasion assay showing the effects of METTL14 and WTAP on invasive ability of CRC cells. ns, not significant, *, p < 0.05, **, p < 0.01. f Quantitative analysis of m6A-methylated XIST in control, siMETTL14–1 and siWTAP cells. Methylated XIST was immunoprecipitated with m6A antibody and then measured by real-time PCR. Equal amount of total RNA was used as input. **, p < 0.01, ***, p < 0.001, ****, p < 0.0001. All data were representative of three independent experiments. Means ± SD were shown. P values were determined by Student’s t-test