Fig. 2

CircSDHC promotes the proliferation and aggressiveness of RCC. a. Target sites of siRNAs used in the knockdown experiment. Both siRNAs target back-splice junction of circPRMT5. b. The knockdown efficiency was measured by qRT-PCR in 786-O and A498 cell lines. c and e. Cell migration and invasion abilities of 786-O and A498 transfected with circSDHC siRNAs or control vector. Number of cells migrated or invaded was determined by counting the cells on five random microscopic field and calculating the mean. Level of migration and invasion were normalized to the control vector group. d and f. Cell proliferation ability of 786-O and A498 transfected with circSDHC siRNAs or control vector. The relative proliferative rate at different time points were normalized to day 0. g. The overexpression plasmid of circSDHC or control vector were transfected into 769P cell line, and expression level of circSDHC was measured by qRT-PCR. h. Cell migration and invasion abilities of 769P transfected with overexpression plasmid or control vector. i. Cell proliferation abilities of 769P transfected with overexpression plasmid or control vector. Data are mean ± SD, n = 3