Fig. 2

Correlation between HRD1 and Vimentin protein expression in breast cancer. a list of the top 6 HRD1-binding protein candidates in MDA-MB-231 cells, analyzed by liquid chromatography-mass spectrometry assay. iBAQ = intensity-based absolute quantification. b Heatmap showing the protein expression of HRD1 and Vimentin assessed with mass spectrometry in breast cancer samples (N = 67) from the Clinical Proteomic Tumor Analysis Consortium (CPTAC). Color key = protein expression Z-score. c Correlation between HRD1 and Vimentin protein expression in breast cancer samples (N = 67) from CPTAC, determined by Pearson’s correlation coefficient. d Representative immunohistochemical staining of HRD1 and Vimentin protein expression in both luminal subtype and TNBC tissues. Scale bar = 50 μm. e Stacked bar charts exhibiting the distribution of breast cancer samples (N = 60) with different expression levels of HRD1 and Vimentin evaluated by the IHC score. 0 (−) and 1 (+) were regarded as low expression, while 2 (++) and 3 (+++) were regarded as high expression. Luminal N = 30, TNBC N = 30. Fisher’s exact test was performed for statistical analysis. f Paired line plots showing the IHC score of HRD1 and Vimentin in matched breast cancer tissue sections (N = 60). P value was calculated as Pearson’s correlation coefficient, showing a reverse correlation of HRD1 and Vimentin