Skip to main content
Fig. 4 | Molecular Cancer

Fig. 4

From: CircNR3C2 promotes HRD1-mediated tumor-suppressive effect via sponging miR-513a-3p in triple-negative breast cancer

Fig. 4

HRD1-mediated Vimentin degradation suppresses TNBC progression and inhibits EMT of breast cancer. a CCK-8 assay showing the optical density at 450 nm of MDA-MB-231 and BT549 cells stably overexpressing HRD1/Vimentin or singly HRD1, suggesting the number of living cells at the indicated timepoints. b Colonies formed by MDA-MB-231 cells stably overexpressing HRD1/Vimentin or singly HRD1 were stained with crystal violet solution, 15 days after seeding. c Cell migration indicated by the scratch width of MDA-MB-231 cells stably overexpressing HRD1/Vimentin or singly HRD1, 0 and 24 h after wound generation. Scale bar = 200 μm. d MDA-MB-231 cells stably overexpressing HRD1/Vimentin or singly HRD1, moving through matrigel in 12 h and adhering to the lower surface of the bottom membrane, were stained with crystal violet solution in transwell invasion assay. Scale bar = 100 μm. e Immunoblotting showing the expression of epithelial marker (E-Cadherin) and mesenchymal markers (Fibronectin, N-Cadherin, Vimentin, TWIST1 and SNAI1) in MCF-7 cells treated with 30 ng/ml of EGF for indicated times. β-actin was used as a loading control. f, g Wound healing and transwell invasion assay showing the migratory and invasive capability of MCF-7 cells treated with EGF for 96 h compared to control cells. Scale bar = 200/100 μm. h Immunoblotting showing the expression of EMT markers in control and HRD1-overexpressing MCF-7 cells upon EGF treatment. β-actin was used as a loading control. i, j Wound healing and transwell invasion assay showing the migratory and invasive capability of control and HRD1-overexpressing MCF-7 cells upon EGF treatment. k Flow cytometry showing the proportion of cancer stem cells (CD44+ and CD133+) in MCF-7 cells with or without HRD1 overexpression upon EGF treatment. The width of scratch, number of colonies and invasive cells per field were measured using ImageJ. Data were represented as means ± S.D. of at least three independent experiments

Back to article page