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Fig. 8 | Molecular Cancer

Fig. 8

From: CircNR3C2 promotes HRD1-mediated tumor-suppressive effect via sponging miR-513a-3p in triple-negative breast cancer

Fig. 8

The circNR3C2/miR-513a-3p/HRD1 axis suppresses tumorigenesis and metastasis by downregulating Vimentin. a Immunoblotting and RT-PCR showing the expression of Vimentin protein and mRNA in control and circNR3C2-overexpressing MDA-MB-231 cells. b CCK-8 assay showing the optical density at 450 nm of MDA-MB-231 and BT549 cells stably overexpressing circNR3C2/Vimentin or singly circNR3C2, suggesting the number of living cells at the indicated timepoints. c Colonies formed by MDA-MB-231 cells stably overexpressing circNR3C2/Vimentin or singly circNR3C2 were stained with crystal violet solution, 15 days after seeding. d Cell migration indicated by the scratch width of MDA-MB-231 cells stably overexpressing circNR3C2/Vimentin or singly circNR3C2, 0 and 24 h after wound generation. Scale bar = 200 μm. e MDA-MB-231 cells stably overexpressing circNR3C2/Vimentin or singly circNR3C2, moving through matrigel in 12 h and adhering to the lower surface of the bottom membrane, were stained with crystal violet solution in transwell invasion assay. Scale bar = 100 μm. f Representative image of whole lungs harvested from BALB/c nude mice 7 weeks after intravenous injection with the indicated groups of MDA-MB-231 cells, showing the metastatic nodules. g Representative hematoxylin and eosin (H&E) staining showing the number of metastatic nodules of mouse lung tissues in Fig. 8 f. Scale bar = 500/200 μm. h Statistical analysis of the lung metastatic nodules in BALB/c nude mice 7 weeks after intravenous injection with the indicated groups of MDA-MB-231 cells. N = 6 per group. i Schematic diagram showing the regulatory mechanisms of the circNR3C2/miR-513a-3p/HRD1 axis on Vimentin protein stability in breast cancer. Data were represented as means ± S.D. of at least three independent experiments

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