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Fig. 4 | Molecular Cancer

Fig. 4

From: CircWAC induces chemotherapeutic resistance in triple-negative breast cancer by targeting miR-142, upregulating WWP1 and activating the PI3K/AKT pathway

Fig. 4

CircWAC acts as a sponge of miR-142 in TNBC. a The expression level of miR-142 in MDA-MB-231 and MDA-MB-468 cells transfected with circWAC or control. b The expression level of miR-142 in MDA-MB-231 and MDA-MB-468 cells transfected with si-circWAC or si-NC. c FISH showed that circWAC and miR-142 were colocalized in the cytoplasm of MDA-MB-231 cells. d The coprecipitated miR-142 was subjected to qRT-PCR to assess circWAC binding by RIP experiments in MDA-MB-231 cells. e MiR-142 and wild-type/mutant circWAC binding site sequences. f Luciferase activity in MDA-MB-231 cells cotransfected with a luciferase reporter vector containing the wild-type or mutant circWAC sequence and the miR-142 mimic or control. g The expression of miR-142 was negatively correlated with circWAC in 90 TNBC patients. h The expression level of miR-142 in TNBC tissues and adjacent nontumor tissues was measured by qRT-PCR. Kaplan-Meier survival analysis of patients with TNBC stratified by the level of miR-142 were used to evaluate OS based on our dataset (N = 90) (I), a METABRIC dataset (N = 203) (J) and a TCGA dataset (N = 97) (K)

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Molecular Cancer

ISSN: 1476-4598