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Fig. 5 | Molecular Cancer

Fig. 5

From: CircWAC induces chemotherapeutic resistance in triple-negative breast cancer by targeting miR-142, upregulating WWP1 and activating the PI3K/AKT pathway

Fig. 5

CircWAC regulates PTX resistance by targeting miR-142. a, b The expression of circWAC and miR-142 in MDA-MB-231 and MDA-MB-468 cells transfected with control + miR-NC, circWAC + miR-NC, control+miR-142 mimic, or circWAC+miR-142 mimic was verified by qRT-PCR. c, d MDA-MB-231 and MDA-MB-468 cells transfected with control + miR-NC, circWAC + miR-NC, control+miR-142 mimic, or circWAC+miR-142 mimic were treated with PTX (concentrations of 0, 5, 10, 15, 20, 25 and 30 μmol/L) for 24 h, and cell viability was evaluated by a CCK-8 assay. e MDA-MB-231 and MDA-MB-468 cells transfected with control + miR-NC, circWAC + miR-NC, control+miR-142 mimic, or circWAC+miR-142 mimic were treated with 20 μM paclitaxel for 24 h, and cell viability was evaluated by a colony formation assay. f MDA-MB-231 and MDA-MB-468 cells transfected with control + miR-NC, circWAC + miR-NC, control+miR-142 mimic, or circWAC+miR-142 mimic were treated with 20 μM paclitaxel for 24 h, and the apoptosis rates were analyzed via flow cytometry. The data are presented as the means±S.D. of at least three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001

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