Fig. 2

CircNEIL3 promotes the proliferation, migration and invasion of PDAC cells in vitro. a RT-qPCR analysis of circNEIL3 and NEIL3 mRNA expression in CFPAC-1 and MiaPaca-2 cells transfected with a lentivirus and circNEIL3 plasmid. b. The growth curves of cells were evaluated by CCK-8 assays after knocking down and overexpressing circNEIL3 in CFPAC-1 and MiaPaca-2 cells. c-d. Colony formation assays were performed to evaluate cell proliferation. e-f. EdU assays of PDAC cells was performed to evaluate cell proliferation. The samples were imaged at 200× magnification. Scale bar = 50 μm. g-h. Transwell assays were performed to assess the migration and invasion abilities of PDAC cells. The samples were imaged at 100× magnification. Scale bar = 100 μm. i-j. Cell migration was assessed using a wound healing assay. The samples were imaged at 100× magnification. Scale bar = 100 μm. All data are presented as the means ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001