Fig. 8

ADAR1 enhances GLI1 RNA editing and functions via the cell cycle and EMT pathway a. Representative Sanger sequencing chromatograms for GLI1 in the indicated cells. The gray box highlights the double A/G peak, labeled with the percentage of edited transcripts assessed as the edited allele burden (G/(G + A) %). b. RIP analyses for GLI1 in CFPAC-1 and MiaPaca-2 cells using an ADAR1 antibody. c-d. Western blot results revealed the expression of ADAR1 and GLI1 in cells transfected with the indicated shRNA vector. e-f. The cell cycle progression was analyzed by flow cytometry after cells were transfected with the indicated plasmids. g. The relative expression of cyclin D1 and downstream cell cycle-related molecules at the protein level in cells transfected with the indicated vectors and shRNAs was determined by western blot analysis. h. The expression of EMT protein markers in the indicated cells was detected by western blot analysis. All data are presented as the means ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001