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Fig. 9 | Molecular Cancer

Fig. 9

From: CircNEIL3 regulatory loop promotes pancreatic ductal adenocarcinoma progression via miRNA sponging and A-to-I RNA-editing

Fig. 9

An Alu-dependent feedback loop involving circNEIL3/miR-432-5p/ADAR1 is correlated with the prognosis of PDAC patients. a-b. The expression levels of circNEIL3 and NEIL3 mRNA in CFPAC-1 and MiaPaca-2 cells transfected with indicated vectors or shRNA were determined by RT-qPCR. c. The level of circNEIL3 expression was analyzed by RT-qPCR. CFPAC-1 and MiaPaca-2 cells were transfected with the miR-432-5p mimics or inhibitor. d-e. RIP assays using an ADAR1 antibody were performed to capture circNEIL3 or NEIL3 mRNA. f-h. Kaplan-Meier survival curves showed the OS of PDAC patients with low vs. high circNEIL3, miR-432-5p or ADAR1 expression. The cutoff value was the median expression of these genes. i-k. Correlation analysis between circNEIL3, miR-432-5p and ADAR1 expression analyzed by RT-qPCR in PDAC tissues (n = 104). l. Schematic diagram illustrating the mechanism by which circNEIL3 promotes PDAC proliferation and metastasis through the circNEIL3/miR-432-5p/ADAR1/GLI1/cell cycle & EMT axis and is regulated by ADAR1 through a negative feedback loop. All data are presented as the means ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001

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Molecular Cancer

ISSN: 1476-4598