Fig. 5

Ablation of YTHDF2 significantly suppresses ovarian cancer cell proliferation and growth in vitro and in vivo. a Stable expression of shRNAs against YTHDF2 in SKOV3 and OVCAR420 cells. The gene knockdown efficiency is verified at both mRNA and protein levels. b YTHDF2 depletion significantly reduces the proliferation rate of SKOV3 and OVCAR420 cells. c YTHDF2 depletion impairs the colony-forming ability of ovarian cancer cells. d YTHDF2 depletion suppresses anchorage-independent growth of ovarian cancer cells. e YTHDF2 depletion promotes ovarian cancer cell apoptosis. f YTHDF2 depletion in SKOV3 cells markedly suppresses ovarian tumor growth in the xenograft mice. g The size of tumors formed in the xenograft mice is monitored every 3 days. (H) YTHDF2 depletion significantly reduces weight of tumors in the xenograft mice. ** P < 0.01. Results were presented as mean ± SD of three independent experiments. *P < 0.05 or **P < 0.01 indicates a significant difference between the indicated groups (i, j, k).