Fig. 1

Dysregulated miRNAs in plasma exosomes of EC patients. a Volcano plot displaying differentially expressed miRNAs between plasma exosome of EC and HC. Small RNA sequencing was performed in plasma exosomes of 25 EC patients and 31 HC controls. There were 49 differentially expressed with p-value < 0.01 (i.e., above the dotted line). b Feature vectors forming the best panel to discriminate two different groups were determined by the random forest algorithm. Six miRNAs (miR-106b-5p, miR-107, miR-15a-5p, miR-139-3p, miR-3615, and miR-574-3p) that indicated higher variable importance in the random forest tree were identified as candidate biomarkers. c Hierarchical clustering analysis of 6 candidate miRNAs roughly divided plasma samples into two distinct groups (EC vs HC). d ROC curves to evaluate the sensitivity and specificity of 6 candidate miRNAs to discriminate EC and HC subjects. e The expression levels of 6 candidate miRNAs in plasma exosomes of EC (n = 25) and HC (n = 31) subjects. f The expression levels of 6 candidate miRNAs in EC tumor tissues and matched para-carcinoma tissues from TCGA (n = 18). HC: healthy controls; PC, para-carcinoma tissues