Fig. 1
From: Pals1 prevents Rac1-dependent colorectal cancer cell metastasis by inhibiting Arf6
Pals1 inhibits cell migration and metastasis of colorectal cancer cells. a and b Representative IHC stainings of Pals1 and E-Cad in healthy colon tissue compared to colorectal cancer specimen. c Survival probability of colorectal cancer patients with high (5,7 FPKM (Fragments Per Kilobase of sequence per Million mapped reads) on average) or low (2,9 FPKM) Pals1 expression. Healthy control samples display on average 7,07 FPKM (not shown). d Representative images of a wound healing assay of HCT116 and HCT116ΔPals1 cell lines and the corresponding quantification (N = 7). e Representative images and quantification of a transwell matrigel invasion assay of HCT116 and HCT116ΔPals1 cell lines (N = 3). f Tumor weight and g average number of metastases found in liver and lung after subcutaneous transplantation of HCT116 and HCT116ΔPals1 in immunodeficient mice after 5 weeks (N = 4 for HCT116wt and HCT116∆Pals1 cells and N = 5 for HCT116∆Pals1 + Pals1 Rescue). h and i Representative pan-Cytokeratin IHC stainings of HCT116ΔPals1 cells colonizing in lung and liver. Scale bars are 20 μm