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Fig. 6 | Molecular Cancer

Fig. 6

From: A novel protein encoded by circMAPK1 inhibits progression of gastric cancer by suppressing activation of MAPK signaling

Fig. 6

Tumor suppressive functions of MAPK1–109aa in GC cell lines. a SGC7901 and MGC803 cells were transfected with circMAPK1 shRNA or circMAPK1 shRNA plus linearized MAPK1–109aa overexpression plasmid. MAPK1–109aa expression was decided by immunoblot. b CCK-8 assay was performed to detect proliferation ability of cells mentioned above. c Colony formation assay was performed to detect proliferation ability of cells mentioned above. d The number of cells is counted with image J. e EdU assay was performed to detect proliferation ability of cells mentioned above. Scale bar: 100 μm. f The number of cells is counted with image J. g The effect of plasmids mentioned above on modulating cell cycle progression was evaluated by flow cytometry assay. h The effect of plasmids mentioned above on cell migration was examined by Transwell assay. Scale bar: 100 μm. Graph represents mean ± SD; *p < 0.05, **p < 0.01, and ***p < 0.001

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