Fig. 5

CircLIFR/MSH2 complex contributes to the CDDP sensitivity via MutSα/ATM-p73 axis in bladder cancer cells. a Co-IP assay was analyzed using T24 and UMUC3 cells lysate which was immunoprecipitated by anti-MSH2 antibody. The precipitate was subjected to western blotting with the antibodies against MSH2, MSH6, MSH3, ATM, ATR, and GAPDH. b Co-IP assay was analyzed using T24 and UMUC3 cells lysate which was immunoprecipitated by anti-ATM antibody. The precipitate was subjected to western blotting with the antibodies against ATM, MSH2, MSH6, MSH3, and GAPDH. c Interaction between MSH2, MSH6, MSH3, and ATM in T24 cells stably transfected with vector or circLIFR, and those cotransfected with scramble, or shMSH2#1. Co-IP experiments with anti-MSH2 antibody were performed, and the precipitate was detected by western blot with the antibodies against MSH2, MSH6, MSH3, ATM, and GAPDH. d T24 cells, which were stably transfected with scramble, shMSH2#1, or shMSH2#2, were treated with 5 μM CDDP for the indicated time. Whole cell lysates were collected for western blot analysis of MSH2, MSH6, ATM, pATM, p73, p63, and GAPDH. e T24 cells, which were stably transfected with scramble, or sh-circLIFR#2, were treated with 5 μM CDDP for the indicated time. Whole cell lysates were collected for western blot analysis of MSH2, MSH6, ATM, pATM, p73, p63, and GAPDH. f Western blot analysis with the indicated antibodies in T24 cells stably transfected with vector or MSH2, and those cotransfected with scramble, or sh-circLIFR#2. g Western blot analysis with the indicated antibodies in T24 cells stably transfected with vector or circLIFR, and those cotransfected with scramble, shMSH1#1, or shMSH1#2. h T24-CDDP cells stably transfected with vector or circLIFR were treated with or without 5 μM CDDP for 24 h, and cell lysates were subjected to western blot analysis with the indicated antibodies. i T24 cells were stably transfected with vector or MSH2 and cotransfected with scramble, or sh-p73. Apoptosis was measured by Annexin-V plus PI staining and FACS analysis. Data were mean ± SD. ***P < 0.01 (Student’s t-test). j T24 cells were stably transfected with vector or circLIFR and cotransfected with scramble, or sh-p73. Apoptosis was measured by Annexin-V plus PI staining and FACS analysis. Data were mean ± SD. ***P < 0.01 (Student’s t-test)