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Fig. 3 | Molecular Cancer

Fig. 3

From: CircMEMO1 modulates the promoter methylation and expression of TCF21 to regulate hepatocellular carcinoma progression and sorafenib treatment sensitivity

Fig. 3

CircMEMO1 Regulates the Level of the TET1/5hmC Axis by Sponging MiR-106b-5p in HCC Cells. a, b miR-106b-5p was abundantly pulled down by a circMEMO1 probe, and miR-106b-5p captured large amounts of circMEMO1 in both Huh-7 and HCCLM3 cells. c Representative seed sequences for miR-106b-5p in the human TET family are shown: 9 base pairs (green), 8 base pairs (red) and 7 base pairs (blue). d HCC cancer cells with different miR-106b-5p expression levels were subjected to western blot analyses for the indicated proteins. e A luciferase assay with the luciferase gene linked to the 3′-UTR of TET1/2 was performed. HEK293 cells were transiently transfected with a combination of pGL3 firefly luciferase reporter plasmids encoding the wild-type 3′-UTR sequences of human TET genes, miR-106b-5p, and a Renilla luciferase reporter for normalization. The data are represented as the mean ± SD (n = 3). f 5hmC-, 5-mC- and DAPI-stained HCC cells expressing different miR-106b-5p levels are shown. Scale bars, 100 μm. g Genomic DNA purified from HCC cells with different miR-106b-5p levels was denatured and neutralized. Global 5hmC levels were then measured by using a dot blot assay with an anti-5hmC antibody. h HCC cancer cells with different circMEMO1 expression levels were subjected to western blot analyses for the indicated proteins. i A dot blot assay with an anti-5hmC antibody was used to detect changes in the 5hmC level in HCC cells with different circMEMO1 expression levels

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